5-26-06 T.H. See Removed tile C023 from storage and mounted into the Level 3 fixture. Tile has a fragment loose on the top near the left end that is still attached but may fall of when the tile is rotated to take the Level 3 top images at 7x & 20x. Had to once again repair the custom light-pipe holder as the top again came unscrewed. Began Depth Profile scans at ~ 10:25 am. To reduce the glare from the fixture in the tile, I mounted the polarizer on the end of the MZ16A with the lightpipe of the ringlight holder in a vertical position. I also relocated the large light source to directly behind the sample instead of from the side. I elevated the light source until it was out of the field of view ~ 3 to 4 inches behind the sample. This resulted in a much more even illumination and much less glare in the tiles toward the Level 3 fixture interface areas. The iris is set at 50% or halfway and the exposure time is for the Camera Control is at 600 ms. Intensity of the light source is set to maximum. (see images for a view of this configuration.) For the reminder of this tile only, the filenames include the letter ācā to indicate that they were all acquired with this, the third light and filter settings. I plan to drop the ācā from all file names on the subsequent tiles. After making the above change, I went back and retook the Depth 1 profile before proceeding on to the Depth 3 profile. Completed Depth Profiles ~ 11:02 am.
Notes By: | ndc\cpgonzal | Date: | 06/07/2010 |