Note Details

Category: Processing

5-26-06 T.H. See Removed tile C023 from storage and mounted into the Level 3 fixture. Tile has a fragment loose on the top near the left end that is still attached but may fall of when the tile is rotated to take the Level 3 top images at 7x & 20x. Had to once again repair the custom light-pipe holder as the top again came unscrewed. Began Depth Profile scans at ~ 10:25 am. To reduce the glare from the fixture in the tile, I mounted the polarizer on the end of the MZ16A with the lightpipe of the ringlight holder in a vertical position. I also relocated the large light source to directly behind the sample instead of from the side. I elevated the light source until it was out of the field of view ~ 3 to 4 inches behind the sample. This resulted in a much more even illumination and much less glare in the tiles toward the Level 3 fixture interface areas. The iris is set at 50% or halfway and the exposure time is for the Camera Control is at 600 ms. Intensity of the light source is set to maximum. (see images for a view of this configuration.) For the reminder of this tile only, the filenames include the letter ā€œcā€ to indicate that they were all acquired with this, the third light and filter settings. I plan to drop the ā€œcā€ from all file names on the subsequent tiles. After making the above change, I went back and retook the Depth 1 profile before proceeding on to the Depth 3 profile. Completed Depth Profiles ~ 11:02 am.